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Objective To analyze the correlation between different types of placental implantation after cesarean section and previous cesarean section.Methods The data of 7 286 pregnant women with second pregnancy after cesarean section from January 2010 to October 2018 were retrospectively analyzed.Among them,842 cases had placental implantation.Five hundred and ninetyseven cases were adhesion type,204 cases were implantation type and 41 cases were penetration type.The relationship between the type of placental implantation in this pregnancy and emergency operation and postpartum hemorrhage during previous cesarean section and the time from this pregnancy was analyzed.Results The total incidence of placental implantation and the incidence of three types of placental implantation in the transfer operation group was higher than that of non-transfer operation group,total:27.4％ (419/1528) vs.7.3％ (423/5 758);adhesion type:18.0％ (275/1 528) vs.5.6％ (322/5 758);implantation type:7.9％ (121/1 528) vs.1.4％ (83/5 758);penetration type:1.5％ (23/1 528)vs.0.3％ (18/5 758),and there were significant differences (P＜0.01).The total incidence of placental implantation and the incidence of three types of placental implantation in postpartum hemorrhage group was higher than that in the non-postpartum hemorrhage group,total:33.9％ (74/218) vs.10.9％ (768/7 068);adhesion type:22.5％ (49/218) vs.7.8％ (548/7 068);implantation type:9.6％ (21/218) vs.2.6％ (183/7 068);penetration type:1.8％ (4/218) vs.0.5％ (37/7 068),and there were significant differences (P＜0.01).There was no statistical significance between the time of pregnancy and the incidence and total incidence of three types of placental implantation (P＞0.05).Conclusions Placental implantation after cesarean section may be closely related to emergency operation and postpartum hemorrhage during the previous cesarean section.Pregnant women with second pregnancy after cesarean section should strengthen the management of pregnancy.
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Objective To study the effect of different grades of corpus luteum blood flow on conservative treatment of unruptured tubal pregnancy??Methods One hundred and fifty?six patients who were diagnosed with unruptured tubal pregnancy in the General Hospital of Northern War Zone and accepted conservative treatment from February 2013 to February 2018 were retrospectively analyzed??The patients were divided into corpus luteum blood level 0 group(0 grade group,36 cases),corpus luteum blood level 1 group (1 grade group,41 cases),corpus luteum blood level 2 group(2 grade group,45 cases),corpus luteum blood level 3 group ( 3 grade group, 34 cases ) according to the corpus luteum blood flow classification in pregnancy??The levels of blood β?HCG,the packet reducing and the curative effect were compared among the four groups??Results The time ofβ?HCG decreased to normal after treatment in 0～3 grade group was (14??4 ±2??7) d, (16??2± 3??9) d, ( 23??3± 4??8) d, (27??0± 4??6) d respectively,there was significant difference among the four groups (F＝5??338,P＝0??039)??The time of mass disappeared completely after treatment in 0－3 grade group was (13??2±2??2) d,( 15??9± 3??0) d,( 21??8± 2??9) d,(24??1± 3??4) d respectively,there was significant difference among the four groups ( F＝4??229,P＝0??043)??The curative rate in 0－3 grade group was 91??7%(33／36),87??8%(36／41),82??2%( 37／45),73??5%( 25／34) respectively,there was significant difference among the four groups(χ2＝4??586,P＝0??041)??There was no significant difference in incidence of adverse reactions among the four groups ( P＞0??05)??Conclusion The different classification of corpus luteum blood flow in pregnancy of unruptured tubal pregnancy have significant influence on the conservative treatment effect??The higher the blood flow classification,the longer treatment time and the worse the curative effect??
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Objective:To compare the effectiveness between three methods for purifying the immunoglobulin of egg yolk(IgY) which are polyethylene glycol (PEG) method, chloroform extraction method and chloroform / PEG method, and to provide basis for obtaining the batch of IgY.Methods:The inactivated vaccine of Vibrio parahemolyticus (V. parahemolyticus) was prepared and the hens were immunized by multi-point intramuscular injection.The eggs were collected and the IgY was purified by PEG method, chloroform extraction method and chloroform/PEG method.The protein extraction rate, the IgY titer and the purity of the antibody which purified by different methods were detected.Furthermore, the operation process, cost and safety of the three methods were analyzed.Results:The protein contents of the extraction belonging three methods from high to low in turn were chloroform extraction method, chloroform/PEG method, and PEG method.There was no significant difference in the antibody titer between three methods, and the tiler of chloroform extraction method was slightly high.The purities of purified antibody from high to low in turn were PEG method, chloroform/PEG method and chloroform method.The PEG method had better security but relatively lower extraction efficiency and higher cost.The chloroform/PEG method had high extraction efficiency and good antibody purity.Conclusion:The PEG method is suitable for a small amount of extraction in the laboratory.The chloroform/PEG method is appropriate for extracting the high quality IgY in a batch as it has high extraction efficiency and good antibody purity.
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Objective To investigate the change and role of nitric oxide (NO), nitric oxide synthase (NOS) in serum of children with Epilepsy.Methods The serum NO and NOS levels of 58 epileptic children and 23 healthy children were measured with enzyme linked immunosorbent assay (ELISA). Results The concentrations of the serum NO [( 5.86? 1.21)?mol/ml] and NOS [( 28.26? 8.49)U/ml] in patient group were significantly higher than those in control group [( 3.78? 0.74)?mol/ml,( 17.86? 4.58)U/ml)]( P